Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Tris-EDTA Buffer | CAS No.:Not specified| MDL No.: MFCD00236359 | 1 L
Tris-EDTA buffer | Purity: 100% | M. W.: Not applicable | CAS No.: Not Specified| MDL No.: MFCD00236359 | 1L
![eMolecules 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925](https://assets.fishersci.com/TFS-Assets/CCG/product-images/NC3454793.JPG-250.jpg)
eMolecules 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Sigma Aldrich Fine Chemicals Biosciences Glycine Buffer Solution | 100ml
Glycine is used as a bulking agent buffers. Glycine at low concentrations prevents pH decrease in solutions. It also stabilizes a protein when present in an amorphous state.
Zymo Research Corporation ZymoPURE™ Binding Buffer (150 ml)
ZymoPURE™ Binding Buffer (150 ml). Assists in binding nucleic acid to ZymoPURE™ columns for purification.
Zymo Research Corporation DNA/RNA Wash Buffer (Concentrate), 24 ml
DNA/RNA Wash Buffer (Concentrate) (24 ml). For use in Quick-DNA™/RNA purification kits. Sold individually or as part of a nucleic acid purification kit.
INNOVAPREP LLC ELUTION FLUID CAN - PBS
FluidPrep Elution Fluid Can - PBS0.075% Tween 20/PBS. Recommended for classical analytical methods.1 can.Each can provides up to 30 extractions.