Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Seracare Life Sciences Inc 10X Phosphate Buffered Saline (PBS), 5 Liter
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled. 5L
Seracare Life Sciences Inc 10X PHOSPHATE BUFFERED SALINE
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled.
Teknova 1M Tris-HCl, pH 9.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
IBI SCIENTIFIC 3P (EDTA) SOLUTION, 100 mL
IBI 0.5MEDTA solution (pH 8.0)is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions. Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.
FUJIFILM Biosciences Iscoves Modified Dulbeccos Med
Iscoves Modified Dulbeccos Medium IMDM is a modification of Dulbeccos Basal Medium Eagle BME that uses high glucose and pyruvate additional amino acids 25mM HEPES selenium and other components IMDM is designed for serumfree growth of hemopoietic cells in culture and supports the growth ofLymphocytes and a variety of other hybrid cells