Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova MES Dilution Buffer, pH 6.5. 1000mL, Sterile 8 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Sigma Aldrich Fine Chemicals Biosciences TAPS BioXtra 99.5 titratio1KG
TAPS BioXtra 99.5 titratio1KG
Bioworld BES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Crescent Chemical Co Inc HYDROXYETHYL)PIPERAZINE-N'-2-E
Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid -Na-salt analytical grade pKa 20 = 7.55. Buffering substance for biochemistry and molecular biology. Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 25249.04 CAS 75277-39-3
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt | 71119-22-7 | MFCD00064350 | 250g
MOPS sodium salt | 99.5% | MW: 231.25 | 71119-22-7 | MFCD00064350 | 250g
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660020.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 25 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a high-quality biochemical reagent used for pH buffering in laboratory research. This 25g package of MOPS is ideal for small-scale laboratory applications that require a stable pH range of 6.5 to 7.9. MOPS is widely used in molecular biology, protein chemistry, and biochemical assays where maintaining precise pH conditions is essential. It is commonly used in enzyme studies, electrophoresis, and protein purification processes. The small packaging size is suitable for labs with lower buffer requirements or for preparing small quantities of buffer solutions for specific experiments.
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660311.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 25 G
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications where maintaining a stable pH is crucial. HEPPS is commonly used in enzyme assays, protein studies, and biochemical experiments, particularly those requiring precise control of pH. The reagent is also utilized in protein purification, electrophoresis, and other applications in molecular biology and biochemistry. Its ability to maintain pH stability at physiological conditions makes it essential for accurate, reliable results in biological research and testing.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660092.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a specialized biochemical reagent used for preparing buffer solutions that maintain a stable pH in laboratory experiments. This 1kg formulation is ideal for high-volume applications, including protein chemistry, enzyme assays, and microbiological studies. The sesquisodium salt form of PIPES offers enhanced buffering capacity and is commonly used in research that requires precise pH control, such as in cell culture, protein purification, and electrophoresis. The buffer solution maintains a stable pH around 7.0, which is essential for maintaining enzyme activity and cellular functions during experiments.
eMolecules Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 10 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659832.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 10 Kg
HEPES, Free Acid (N-(2-Hydroxyethyl) Piperazine-N'-(2-Ethanesulfonic Acid)) is a biochemical reagent that provides highly stable pH conditions for biological and chemical research. The 10kg bulk size is designed for large laboratories that require significant quantities of HEPES for buffer preparation and other research applications. HEPES is widely used in cell culture, protein assays, and enzyme reactions due to its minimal interference with biological processes. It is ideal for applications where precise pH control is essential, such as in molecular biology, immunology, and microbiology. The free acid form of HEPES is highly soluble in water, providing a versatile and effective buffer for a variety of laboratory procedures.
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661641.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) is a biochemical reagent used for pH buffering in biochemical applications. This 1 kg volume is ideal for laboratories requiring large quantities of MES buffer for a variety of experiments. MES is commonly used in biochemical research, especially in enzyme assays, cell culture, and protein purification. It maintains a stable pH range, crucial for experiments that need precise and consistent conditions. The MES buffer ensures minimal interference in reactions, making it ideal for applications such as electrophoresis and chromatography. MES Monohydrate is a reliable and effective reagent used in a wide range of scientific studies, particularly in molecular biology and biochemistry.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 1 KG
MOPS sodium salt is a buffering agent used in biology. It maintains the pH of mammalian cell culture media and significantly improves the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH in mammalian cell culture media.
- Improves thermal stability of BSA.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 100G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 100G
Bioworld ACES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.